منابع مشابه
The ODC 3′-Untranslated Region and 5′-Untranslated Region Contain cis-Regulatory Elements: Implications for Carcinogenesis
It has been hypothesized that both the 3'-untranslated region (3'UTR) and the 5'-untranslated region (5'UTR) of the ornithine decarboxylase (ODC) mRNA influence the expression of the ODC protein. Here, we use luciferase expression constructs to examine the influence of both UTRs in keratinocyte derived cell lines. The ODC 5'UTR or 3'UTR was cloned into the pGL3 control vector upstream or downst...
متن کاملDetecting Untranslated Content for Neural Machine Translation
Despite its promise, neural machine translation (NMT) has a serious problem in that source content may be mistakenly left untranslated. The ability to detect untranslated content is important for the practical use of NMT. We evaluate two types of probability with which to detect untranslated content: the cumulative attention (ATN) probability and back translation (BT) probability from the targe...
متن کاملTethered function assays using 3' untranslated regions.
Proteins that regulate mRNA metabolism are often bipartite: an RNA binding activity confers substrate specificity, and a "functional" domain elicits the biological outcome. In some cases, these two activities reside on different polypeptides that form a complex on the mRNA. Regardless, experimental separation of RNA binding from function facilitates analysis of both properties, liberating each ...
متن کاملDom34 Rescues Ribosomes in 3′ Untranslated Regions
Ribosomes that stall before completing peptide synthesis must be recycled and returned to the cytoplasmic pool. The protein Dom34 and cofactors Hbs1 and Rli1 can dissociate stalled ribosomes in vitro, but the identity of targets in the cell is unknown. Here, we extend ribosome profiling methodology to reveal a high-resolution molecular characterization of Dom34 function in vivo. Dom34 removes s...
متن کاملRegulatory 3′ Untranslated Regions of Bacterial mRNAs
The untranslated regions (UTRs) of mRNA contain important features that are relevant to the post-transcriptional and translational regulation of gene expression. Most studies of bacterial UTRs have focused on the 5'regions; however, 3'UTRs have recently emerged as a new class of post-transcriptional regulatory elements. 3'UTRs were found to regulate the decay and translation initiation in their...
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ژورنال
عنوان ژورنال: Science
سال: 2010
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.327.5964.394-a